Anti-Dishevelled 3 Antibody

Dishevelled 3 (DVL3) is a cytoplasmic phosphoprotein central to the Wnt signaling cascade, transmitting signals from Frizzled family receptors to downstream effectors that govern β-catenin stability, planar cell polarity, and calcium-sensitive pathways. This rabbit polyclonal reagent from Antibodies.com was raised against the DVL3 protein and is supplied at 1 mg/ml in PBS buffer containing 0.1% sodium azide and 50% glycerol, delivered in a 100 µl vial format.

Validated for western blot detection across human, mouse, and rat sample lysates, the unconjugated antibody accommodates chemiluminescent, fluorescent, or enzyme-based readout when paired with appropriate HRP- or fluorophore-conjugated secondary reagents. Investigators studying canonical Wnt/β-catenin signaling, embryonic axis specification, or DVL3's documented involvement in oncogenesis (including activating mutations reported in lung adenocarcinoma) commonly use this reagent to probe endogenous expression, phosphorylation status, and pathway activation in cell culture and tissue-derived samples.

The 50% glycerol cryoprotectant allows the antibody to remain liquid at -20 °C for long-term storage, eliminating ice-crystal damage to the protein. For best results, aliquot the vial upon first use to limit freeze-thaw cycles across extended studies. For western blot, a working concentration of 0.5 to 2 µg/ml offers a reasonable starting range, though optimal dilutions should be empirically determined for each lysate preparation, membrane type, and imaging system.