Anti-DIAPH1 Antibody

Offered through the Antibodies.com catalogue, this affinity-purified polyclonal reagent is raised in rabbit against recombinant full-length human DIAPH1 (diaphanous homolog 1), enabling detection of endogenous DIAPH1 protein in both denaturing and fixed-tissue workflows. The antibody is supplied as a 50 µl liquid formulation at 1 mg/ml in PBS containing 0.1% sodium azide and 50% glycerol, and is unconjugated for direct use with secondary detection systems. Recommended applications are Western blot (WB) and immunohistochemistry (IHC), and the reagent has been validated to cross-react with Human, Mouse, and Rat samples. Reported molecular weights are approximately 141 and 150 kDa, consistent with the full-length DIAPH1 isoform, and the antibody is purified by antigen-affinity chromatography to greater than 95% purity by SDS-PAGE. For best stability, the vial should be stored frozen long term in its glycerol-containing buffer, aliquoted to limit repeated freeze–thaw cycles. Researchers working on actin cytoskeleton regulation, formin signalling, or auditory/inner-ear biology pathways linked to DIAPH1 (DFNA1/DRF1/hDIA1) will find this polyclonal a flexible primary reagent for WB and IHC.