Anti-DIAPH1 Antibody

Rabbit polyclonal antiserum raised against DIAPH1 (Diaphanous Related Formin 1), a formin-family protein that nucleates and elongates linear actin filaments through its conserved FH2 domain. The antibody is supplied by Antibodies.com as an unconjugated liquid reagent in a 100 µl vial, suited for western blot and immunohistochemistry workflows on human, mouse, and rat samples. DIAPH1 participates in cytoskeletal remodeling, stress fiber assembly, microtubule stabilization, and vesicle trafficking, and the formin is implicated in hereditary hearing loss (DFNA1), platelet cytoskeletal defects, and metastatic invasion in several tumor contexts. Researchers apply this reagent to confirm DIAPH1 expression across cell lines and tissue sections, to map subcellular distribution at the cell cortex and along microtubule bundles, and to probe formin activity changes following Rho-GTPase, Src-family kinase, or integrin signaling perturbations. Storage at 4 °C is recommended for short-term use, with aliquoting advised to limit freeze-thaw cycles and preserve polyclonal titer over time.