Anti-DHCR7 Antibody

Raised in rabbits against human 7-dehydrocholesterol reductase, this polyclonal IgG reagent from Antibodies.com targets the terminal enzyme of the cholesterol biosynthesis pathway. DHCR7 catalyzes the reduction of 7-dehydrocholesterol to cholesterol, and loss-of-function mutations in the gene are linked to Smith-Lemli-Opitz syndrome, making this antibody a useful reagent for studies of sterol metabolism, neurodevelopment, and hepatic cholesterol homeostasis.

Supplied as an unconjugated preparation in a 100 µl vial, the antiserum is validated for Western blot detection of the human target. Because the antibody is unconjugated and isotype IgG, researchers can pair it with their preferred HRP-, fluorophore-, or biotin-conjugated secondary reagent. The polyclonal format enables recognition of multiple epitopes on the reductase, which can improve signal in western blot applications where single monoclonal clones may fail.

Key product details include rabbit host, polyclonal clonality, IgG isotype, unconjugated conjugate, and confirmed reactivity against human DHCR7. Recommended storage follows standard antibody handling conditions to preserve activity across multiple freeze-thaw cycles when small working aliquots are prepared.

Common research uses include Western blot analysis of DHCR7 protein expression in human cell lines and tissue lysates, characterization of cholesterol biosynthesis regulation, and investigation of Smith-Lemli-Opitz syndrome in disease models.