Anti-DGKB Antibody

The rabbit polyclonal anti-DGKB reagent from Antibodies.com is an unconjugated IgG preparation raised against diacylglycerol kinase beta, the type II isoform of the enzyme family that converts diacylglycerol into phosphatidic acid during lipid second-messenger signaling. Supplied at 200 µl per vial, the antibody is validated for western blot detection of endogenous DGKB in mouse and rat samples, supporting neuroscience and signal-transduction workflows where DAG kinase activity is being mapped across tissues or developmental stages. Because it is polyclonal, the reagent binds multiple epitopes on the target, which improves tolerance to minor denaturation differences between sample preparations and gives more robust signal on reduced gels loaded with whole-cell lysates or brain homogenates. The unconjugated format leaves detection chemistry to the user, so the antibody can be paired with HRP-conjugated secondary reagents for chemiluminescent western blot work or with fluorophore-labeled secondaries for multiplex imaging. Reactivity is confirmed against murine and rat DGKB, while predicted cross-reactivity with other diacylglycerol kinase isoforms is limited, a useful specificity profile when researchers want to distinguish DGKB from closely related alpha, gamma, or zeta family members. Store refrigerated at 2-8 degrees C in working aliquots to limit freeze-thaw cycles and preserve binding titer over the shelf life of the reagent. Each lot is quality controlled for application performance, and the 200 µl pack size offers enough material for multiple western blot membranes and downstream validation studies.