Anti-DGK-α Antibody

This rabbit polyclonal reagent from Antibodies.com is raised against diacylglycerol kinase alpha (DGK-α) and affinity-purified from rabbit antiserum using an epitope-specific immunogen, yielding approximately 95% purity by SDS-PAGE. The unconjugated IgG preparation detects endogenous levels of the DGK-α protein in Human, Mouse, and Rat samples, providing reactivity across species for comparative lipid-signaling studies in cell lysates and human tissue.

The immunogen corresponds to recombinant full length Human DGK-α, a member of the diacylglycerol kinase family that converts the second messenger diacylglycerol into phosphatidic acid, attenuating protein kinase C activity. On western blot, the target runs at an apparent molecular weight of approximately 83 kDa, consistent with the canonical 80 kDa DGK-α isoform.

Supplied at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol as a cryoprotectant, the 100 µl vial format can be diluted directly into standard immunodetection buffers. Applications validated for this antibody include western blot (WB) and immunohistochemistry (IHC), supporting protein expression profiling and tissue distribution studies. Polyclonal recognition of multiple epitopes on DGK-α helps researchers probe T-cell receptor signaling, neuronal lipid metabolism, and cancer pathways where DGK activity is implicated. The glycerol content protects the antibody from freeze-thaw damage during refrigerated storage.