Anti-DGCR8 Antibody

DGCR8 (DiGeorge Syndrome Critical Region 8) is the essential RNA-binding cofactor of the nuclear Microprocessor complex, where it partners with the RNase III enzyme Drosha to cleave primary microRNA (pri-miRNA) transcripts into ~70-nt precursor hairpins that are later processed into regulatory miRNAs of about 22 nucleotides. This unconjugated rabbit polyclonal IgG reagent from Antibodies.com was raised against an epitope within human DGCR8 and detects the endogenous protein at its predicted molecular weight of approximately 110 kDa across human, mouse, and rat samples. Validated for western blot, immunohistochemistry, immunofluorescence, immunoprecipitation, and flow cytometry, the antibody is supplied as a 100 µl liquid vial. The polyclonal format provides recognition of multiple epitopes on the full-length target, which is robust on denatured lysates and on fixed tissue sections where only a subset of epitopes may remain accessible. Researchers use it to investigate nuclear miRNA biogenesis, the 22q11.2 hemizygous deletion underlying DiGeorge/velocardiofacial syndrome, neural crest and cardiac outflow tract development, and altered microRNA processing in tumor and stem-cell biology. Upon receipt, aliquot and store at -20°C, avoiding repeated freeze-thaw cycles; the vial is shipped refrigerated at 4°C.