Anti-DGAT1 Antibody

DGAT1 (diacylglycerol acyltransferase 1) catalyzes the final, rate-limiting step in mammalian triglyceride biosynthesis, transferring a fatty acyl group from acyl-CoA onto diacylglycerol. As an integral endoplasmic reticulum membrane protein, DGAT1 is a focal target in research on adipogenesis, hepatic steatosis, insulin sensitivity, and lipid droplet biology.

This unconjugated rabbit polyclonal reagent from Antibodies.com is supplied as 100 µl of antiserum and is validated for Western blot (WB) and immunohistochemistry (IHC) across human, mouse, and rat samples. The unconjugated format gives researchers flexibility to pair the antibody with a reporter-conjugated secondary antibody selected for their detection platform.

In Western blot applications, the antibody detects the endogenous DGAT1 band at approximately 55 kDa in whole-cell and tissue lysates. For IHC work, antigen retrieval conditions and primary antibody titration should be optimized empirically against formalin-fixed, paraffin-embedded sections. For long-term storage, aliquot on first use and store refrigerated to preserve reagent activity across multiple freeze-thaw cycles.