Anti-DFFB Antibody

DNA Fragmentation Factor subunit beta (CAD) is the caspase-activated endonuclease that drives oligonucleosomal DNA cleavage during apoptosis. This unconjugated rabbit polyclonal antibody from Antibodies.com binds DFFB directly and is validated for Western blot detection across Human, Mouse, and Rat samples. The reagent is supplied as 100 µl of a 1 mg/ml formulation in phosphate buffered saline (pH ~7.3) containing 0.05% sodium azide as preservative, and can be diluted into standard Laemmli or RIPA lysate workflows. Researchers use it to monitor full-length CAD expression, to follow caspase-3–mediated liberation of the endonuclease from its ICAD (DFFA) chaperone, and to compare apoptotic responses in oncology, neurodegeneration, and toxicology models. Aliquot upon receipt and store at -20°C in single-use fractions to limit freeze-thaw cycles and preserve binding activity across repeated Western blot experiments.