Anti-DDX5 Antibody

Manufactured by Antibodies.com, this rabbit polyclonal reagent is raised against DDX5 (DEAD-box helicase 5, also known as p68 / HLR1 / G17P1) and is intended for the detection of endogenous total DDX5 protein in human, mouse, and rat samples. The immunogen is a recombinant human DDX5 protein, and the antibody is purified by affinity chromatography using the immunogen prior to formulation. The host species is rabbit, the clonality is polyclonal, the immunoglobulin class is IgG, and the preparation is supplied unconjugated, allowing users to couple it to their preferred reporter or label. The product is provided as a 50 µL aliquot at a working concentration of 1.0 mg/mL in phosphate-buffered saline (pH 7.4, without Mg²⁺ and Ca²⁺), containing 150 mM NaCl, 0.02% sodium azide as a preservative, and 50% glycerol as a cryoprotectant. Verified applications on the supplier datasheet are Western blot (WB) and immunohistochemistry (IHC); recommended dilutions typically fall within WB1:500–1:2,000 and IHC 1:50–1:200, although optimal titres should be determined empirically. The antibody is shipped at 4 °C and should be aliquoted and stored at –20 °C upon arrival, with repeated freeze/thaw cycles avoided to preserve activity. It is intended for research use only and is not validated for diagnostic or therapeutic applications.