Anti-DDX43 Antibody

This polyclonal antibody from Antibodies.com targets DDX43, a DEAD-box RNA helicase also known as HAGE (Helicase-Antigen) that is normally restricted to testicular tissue but becomes aberrantly re-expressed across multiple solid tumor types. Raised in rabbit and supplied as an unconjugated 100 µL aliquot, the reagent is validated for Western blot and immunohistochemistry workflows against human samples. Investigators apply it to characterize DDX43 abundance in breast carcinoma, lung carcinoma, head and neck squamous cell carcinoma, and colorectal tissue specimens, where elevated levels have been linked to proliferative signaling and tumor progression. The antibody also supports preclinical research into HAGE as a candidate target for cancer immunotherapy and tumor-associated antigen vaccine design. Affinity purification of the polyclonal preparation enables recognition of multiple epitopes across the full-length DDX43 protein, useful when probing cell lysates or formalin-fixed paraffin-embedded sections that may contain minor isoforms. For handling, aliquot upon arrival, store at -20 °C, and limit freeze-thaw cycles to preserve binding activity. The unconjugated format allows researchers to pair the primary antibody with their preferred HRP, fluorophore, or biotin-based secondary detection system, supporting flexibility across immunofluorescence, chromogenic IHC, and standard Western blot protocols.