Anti-DDX3X Antibody [Assigned #A10099]

DDX3X is an ATP-dependent RNA helicase encoded on the X chromosome that participates in translation initiation, RNA splicing, stress granule assembly, and innate immune sensing of viral RNA, and this mouse monoclonal IgG reagent from Antibodies.com provides a sensitive tool for tracking the protein across multiple experimental platforms. The unconjugated antibody is supplied as a 200 µl liquid vial and is validated for western blot, immunocytochemistry, and immunoprecipitation workflows across human, mouse, rat, and monkey samples, supporting studies of tumor translation control, viral host-factor biology, and the neurodevelopmental DDX3X syndrome. For storage, the antibody should be kept refrigerated at 4 °C in working aliquots to limit freeze-thaw cycles and preserve binding activity over months of use. Researchers commonly deploy this reagent to immunoprecipitate DDX3X from cytoplasmic lysates for mass spectrometry interactome work, to track its relocalization into stress granules after arsenite or hippuristanol treatment, and to confirm CRISPR knockout clones by immunoblot. Cross-reactivity with the listed species reflects conserved epitopes within the helicase core, while use on fixed cells for ICC commonly requires methanol fixation to retain the nuclear and cytoplasmic staining characteristic of this RNA-binding protein.