Anti-DDX3X Antibody

Raised in rabbit and validated for western blotting, immunohistochemistry and immunocytochemistry/immunofluorescence, this polyclonal IgG reagent from Antibodies.com targets the DEAD-box helicase 3, X-linked (DDX3X) protein in human, mouse and rat samples. The antibody detects endogenous levels of total DDX3X and was generated against a recombinant human DDX3X immunogen, then affinity-purified to a working concentration of 1.0 mg/mL in phosphate-buffered saline (pH 7.4) containing 150 mM NaCl, 0.02% sodium azide and 50% glycerol. It is supplied as a 50 µL liquid, unconjugated aliquot that is shipped at 4 °C and should be aliquoted and stored long-term at –20 °C, with repeated freeze/thaw cycles avoided. The reagent performs reliably in WB on whole-cell lysates, in IHC on formalin-fixed tissue sections and in ICC/IF for subcellular localization studies, making it a versatile primary antibody for laboratories investigating RNA helicase function, stress-granule biology and translational control across multiple model systems. As an affinity-purified polyclonal, the reagent offers broad epitope coverage for consistent detection of the target, and it is intended for research use only, not for diagnostic or therapeutic applications.