Anti-DDX3 Antibody

Targeting the DEAD-box RNA helicase DDX3, this rabbit polyclonal antibody from Antibodies.com is supplied as 100 µl at 1 mg/ml in a vial of PBS with 0.1% sodium azide and 50% glycerol. DDX3 functions in translation initiation, stress granule assembly, and Wnt/β-catenin signaling, and serves as a host factor exploited by several RNA viruses, making this reagent relevant to studies of post-transcriptional gene regulation, oncogenic signaling, and viral host-pathogen interactions. The unconjugated format allows flexible pairing with fluorescent or enzyme-conjugated secondary antibodies for detection. Validated applications span western blot for endogenous expression analysis in human, mouse, and rat samples, immunohistochemistry for tissue localization, immunofluorescence for visualizing subcellular distribution including cytoplasmic RNA granules, immunoprecipitation of native DDX3-containing ribonucleoprotein complexes, and RNA immunoprecipitation for mapping DDX3-associated transcripts. The polyclonal composition recognizes multiple epitopes across the target, improving the likelihood of capture under denaturing or partially denatured conditions. Short-term storage at 4°C is appropriate; aliquot and store at -20°C for longer periods and limit freeze-thaw cycles to preserve binding activity.