Anti-DDDDK Tag Antibody

The DDDDK tag (also widely known as the FLAG tag) is a short hydrophilic octapeptide fused to recombinant proteins to enable purification and detection. This mouse polyclonal antibody recognizes the DDDDK epitope and is supplied as a 200 µl liquid format from Antibodies.com.

Validated across multiple workflows, the reagent supports western blot detection of DDDDK-tagged fusion proteins, immunohistochemistry on fixed tissue sections expressing tagged constructs, immunofluorescence imaging for subcellular localization, and immunoprecipitation to pull down tagged protein complexes from cell lysates. The unconjugated IgG formulation gives researchers flexibility to pair the antibody with custom secondary conjugates and label chemistries suited to their detection platform.

Because the DDDDK tag is appended to a recombinant protein of interest, this reagent is routinely used to confirm expression, monitor purification yields, and characterize protein-protein interactions in expression systems ranging from bacterial lysates to mammalian cell lines. Polyclonal reactivity broadens recognition of the tag in different protein contexts, helping reduce signal loss when the tag is partially occluded by folding partners.

Storage guidance for liquid polyclonal IgG preparations generally recommends aliquoting on receipt to limit freeze-thaw cycles and storing at -20 °C in working volumes for active use. For short-term bench work, keep the vial refrigerated and protect from light to preserve antibody activity.