Anti-DDDDK Tag Antibody

The DDDDK epitope, also widely known as the FLAG tag (sequence DYKDDDDK), is a short peptide engineered onto recombinant proteins to enable affinity purification and sensitive detection. This unconjugated mouse polyclonal IgG reagent binds that tag and is validated across four core workflows: Western blot analysis of cell lysates, immunohistochemistry on fixed tissue sections, immunofluorescence imaging of transfected cells, and immunoprecipitation of FLAG-tagged complexes from mammalian, insect, and bacterial expression systems.

Supplied as 100 µl of polyclonal antibody, it recognizes N-terminal, C-terminal, and internal FLAG fusions alike, making it useful for confirming expression levels, mapping subcellular localization, pulling down interacting partners, and screening engineered stable cell lines. Researchers working on tagged constructs can apply it directly to standard blotting and staining protocols without secondary-conjugation steps, since the reagent itself is supplied unconjugated.

For routine use, refrigerate the vial; for longer retention, prepare aliquots and freeze, limiting repeated freeze-thaw cycles to preserve antibody activity. The reagent is intended for research use only and forms part of the primary antibody portfolio offered by Antibodies.com for tag-based detection in cell biology and protein biochemistry workflows.