Anti-DDB1 Antibody

DDB1 (Damage-specific DNA Binding Protein 1) is a ~127 kDa adapter subunit of the CRL4 (CUL4-DDB1) ubiquitin ligase complex and an essential early sensor in global-genome nucleotide excision repair, where it partners with DDB2 to recognize ultraviolet-induced photoproducts and initiate substrate ubiquitination. This unconjugated rabbit polyclonal reagent from Antibodies.com enables detection of endogenous human DDB1 across western blot, immunohistochemistry, and immunofluorescence workflows, giving researchers a flexible tool for studying DNA damage response, xeroderma pigmentosum, and CRL4-dependent regulation of cell cycle and chromatin-associated targets.

Each vial contains 100 µl of affinity-purified antibody raised in rabbit against human DDB1, supplied unconjugated so it can be paired with HRP-, biotin-, or fluorophore-conjugated secondaries depending on assay design. For best performance, store the reagent at -20 °C in single-use working aliquots, avoiding repeated freeze-thaw cycles; once thawed, an active aliquot may be kept refrigerated for routine use. The polyclonal format offers broad epitope coverage, supporting consistent band detection at the expected molecular weight in western blot and nuclear-localized signal in immunofluorescence and IHC on fixed cells and frozen tissue sections.

Validation data and recommended dilution ranges are provided with the lot to help users optimize the reagent for their specific sample type, whether working with cultured human cell lines, patient-derived tumor sections, or xenograft tissue lysates. Researchers investigating CRL4 E3 ligase biology, nucleotide excision repair capacity, or DDB1-associated viral hijacking mechanisms rely on Antibodies.com primary antibodies for reproducible target detection in research use only applications.