Anti-DDB1 Antibody

DDB1 (DNA Damage-Binding Protein 1) serves as a core adapter of the CUL4-DDB1 E3 ubiquitin ligase complex and participates in the recognition of UV-induced DNA lesions during nucleotide excision repair. This unconjugated mouse monoclonal reagent from Antibodies.com targets a conserved epitope on DDB1 and has been validated for Western blot detection across human, mouse, rat, and monkey sample types.

The antibody is supplied as 100 µl of purified immunoglobulin in a 0.1 M Tris-Glycine buffer at pH 7.4, containing 150 mM NaCl, 0.2% sodium azide, and 50% glycerol. The cryoprotectant formulation allows the vial to be stored at -20 degrees C in single-use aliquots, with protection from light recommended to preserve binding activity across multiple freeze-thaw cycles. Each lot is produced from mouse hybridoma culture and purified to remove serum-derived contaminants before final formulation.

For Western blot workflows, researchers typically resolve 20-50 µg of total protein per lane and detect endogenous DDB1 at the expected molecular weight of approximately 127 kDa using standard chemiluminescent detection with secondary antibodies raised against mouse IgG. The single-use vial format and unconjugated presentation make this reagent appropriate for downstream immunoprecipitation studies of CUL4-associated complexes and characterization of DNA damage response pathways.