Anti-DCTN2 Antibody - Identical to Abcam (ab185928)

Offered by Antibodies.com as a Primary Antibody reagent, this rabbit polyclonal IgG antibody recognises DCTN2 (also referred to as p50 dynamitin) and is supplied unconjugated in liquid form. It is validated for Western blot (WB) and immunocytochemistry/immunofluorescence (ICC/IF) and reacts with Human and Rat samples, with the immunogen being a recombinant fusion protein corresponding to amino acids 50–320 of human DCTN2 (NP_006391.1). The target protein has a molecular weight of 44 kDa and the antibody, purified by affinity chromatography, is formulated in Phosphate Buffered Saline pH 7.3 with 50% glycerol and 0.02% sodium azide. Recommended working dilutions are 1:500–1:2,000 for WB and 1:20–1:50 for ICC/IF. The product is shipped at 4°C and should be aliquoted and stored at -20°C, with freeze/thaw cycles avoided. Published validation includes a Western blot of MCF-7 cell extracts and immunofluorescence imaging of U2OS cells with DAPI nuclear counterstain.