Anti-DCTN2 Antibody

Produced by Antibodies.com as part of their Dynactin-2 (DCTN2) antibody range, this polyclonal reagent (catalog A35241) is raised in rabbit and supplied at 1.0 mg/mL in a 50 µL volume. The antibody was generated against a recombinant human DCTN2 immunogen and purified by affinity chromatography using the same immunogen, yielding an IgG preparation that is unconjugated and free of carrier proteins beyond standard buffer components. It is formulated in phosphate-buffered saline at pH 7.4 (without Mg²⁺ or Ca²⁺), containing 150 mM sodium chloride, 0.02% sodium azide, and 50% glycerol, and is shipped at 4 °C for short-term transit. For long-term use, the supplier recommends aliquoting upon arrival and storing at -20 °C, while avoiding repeated freeze/thaw cycles. The reagent specifically recognizes the endogenous total DCTN2 protein, also known as DCTN-50, dynactin complex 50 kDa subunit, p50 dynamitin, or 50 kDa dynein-associated polypeptide, and has been validated by Antibodies.com for Western blotting (WB) and immunocytochemistry/immunofluorescence (ICC/IF). Reactivity is listed on the supplier product page for human and rat samples, and independent antibody compendia extend this to mouse DCTN2 as well. Validation images on the supplier page additionally show use in immunohistochemistry on paraffin-embedded human kidney tissue at a 1:200 dilution. Each vial provides sufficient material for routine immunoblot and immunostaining workflows in laboratories investigating cytoplasmic dynein/dynactin function, microtubule-based transport, vesicle trafficking, mitotic spindle dynamics, or related cytoskeletal processes.