Anti-DCK Antibody

DCK (deoxycytidine kinase) is the rate-limiting enzyme in the deoxyribonucleoside salvage pathway and catalyzes the phosphorylation of deoxycytidine as well as clinically important nucleoside analog prodrugs such as gemcitabine, cytarabine, and decitabine. This unconjugated rabbit polyclonal reagent from Antibodies.com was raised against DCK and is supplied at 100 µl per vial, ready for use in standard immunodetection workflows.

The antibody has been validated for Western blot, immunohistochemistry, and immunofluorescence applications, and it cross-reacts with human, mouse, and rat samples, which makes it useful for both clinical pathology specimens and rodent model tissues. Researchers studying nucleoside metabolism, antimetabolite drug activation, leukemia biology, or combinations of deoxycytidine kinase expression with chemosensitivity profiles will find the reagent compatible with paraffin-embedded sections, frozen tissue, and cultured cell preparations.

Being a polyclonal reagent, the antibody recognizes multiple epitopes on DCK, which can be advantageous when detecting low-abundance targets or when working with samples in which the protein may be partially degraded. Pair it with appropriate secondary antibodies for chromogenic or fluorescent detection depending on the application, and include positive controls such as proliferating lymphocyte populations or gemcitabine-treated cell lines to confirm specific staining.