Anti-DARPP-32 Antibody

Produced by Antibodies.com as a rabbit polyclonal reagent, this unconjugated primary antibody targets DARPP-32 (dopamine- and cAMP-regulated neuronal phosphoprotein, 32 kDa), a key intracellular signaling molecule encoded by PPP1R1B that is densely expressed in striatal medium spiny neurons and serves as a widely used marker for dopaminergic pathway studies.

The reagent is supplied as a 100 µl liquid vial and is validated for western blot, immunohistochemistry, and ELISA applications, with demonstrated reactivity against human, mouse, rat, and monkey samples. The polyclonal format recognizes multiple epitopes on DARPP-32, which is useful when probing both the full-length phosphoprotein and its major post-translationally modified forms at Thr34 and Thr75, the two regulatory sites that integrate cAMP/PKA and CDK5 signaling cascades.

In the laboratory, researchers apply this antibody in models of Parkinson's disease, Huntington's disease, schizophrenia, drug addiction, reward circuitry, and L-DOPA-induced dyskinesia, where DARPP-32 phosphorylation state reflects dopaminergic activity. The protein is also studied in non-neural contexts, including renal and adrenal tissue. The unconjugated rabbit host antibody pairs with standard anti-rabbit secondary reagents conjugated to HRP, fluorophores, or biotin for in vitro detection.

For routine handling, store the vial refrigerated for short-term use and freeze in single-use aliquots at -20°C for long-term stability, avoiding repeated freeze-thaw cycles to preserve binding activity. Each vial is intended for research use only.