Anti-DARPP-32 Antibody

Manufactured by Antibodies.com, this affinity-purified rabbit polyclonal reagent targets DARPP-32 (Dopamine- and cAMP-regulated neuronal phosphoprotein, 32 kDa) and is supplied at 50 µl per vial at a concentration of 1.0 mg/mL. The unconjugated antibody is formulated in phosphate-buffered saline (pH 7.4) containing 150 mM NaCl, 0.02% sodium azide and 50% glycerol as Rabbit IgG, and is validated for Western blot (WB), immunohistochemistry (IHC) and immunocytochemistry/immunofluorescence (ICC/IF). It detects endogenous levels of total DARPP-32 protein in human, mouse and rat samples, and was raised against a synthesized non-phosphopeptide derived from the human DARPP-32 sequence around the threonine 75 phosphorylation site (A-Y-T(p)-P-P). The antibody is purified by affinity chromatography using the epitope-specific immunogen and is shipped on cold packs at 4 °C; upon arrival it should be aliquoted and stored at −20 °C, with repeated freeze/thaw cycles avoided to preserve activity. Suitable for neuroscience and signal-transduction research, the product is offered through the Antibodies.com catalogue as part of their Primary Antibodies range, with typical dispatch from St. Louis, MO within 6–9 business days.