Anti-DAPK1 Antibody

DAPK1 (Death-Associated Protein Kinase 1) is a calcium/calmodulin-dependent serine/threonine kinase with established roles in programmed cell death, autophagy regulation, and tumor suppression signaling, and is widely studied as a regulator of apoptotic and stress-response pathways.

This rabbit polyclonal reagent from Antibodies.com is raised against DAPK1 and supplied as 100 µl of unconjugated antiserum, validated for Western blot and immunohistochemistry workflows. The antibody is reactive with human, mouse, and rat samples, supporting translational studies that compare DAPK1 expression across rodent model organisms and human patient tissue.

Researchers apply the reagent to investigate apoptotic signaling in neurodegeneration, ischemic injury, and oncology, where DAPK1 phosphorylation status and total expression are quantified by immunoblotting or visualized in fixed tissue sections. As a polyclonal reagent, it recognizes multiple epitopes on DAPK1, which is advantageous for detecting low-abundance target protein or capturing the full range of isoforms present in denatured lysates.

The unconjugated format gives laboratories flexibility to conjugate the antibody to HRP, biotin, or fluorophores in-house according to their chosen detection chemistry. Storage at -20 °C in small aliquots is recommended to limit freeze-thaw cycles and preserve binding activity across the reagent's working lifetime.