Anti-DAO Antibody

Primary antibodies from Antibodies.com directed against diamine oxidase support studies of histamine catabolism, mucosal biology, and amine metabolism. This unconjugated rabbit polyclonal reagent recognizes human DAO and is validated for western blot, immunohistochemistry, and immunofluorescence, giving labs flexibility for protein confirmation in lysates as well as spatial mapping in fixed tissue.

Each 100 µL vial supplies sufficient volume for multiple experiments, and the polyclonal format provides broader epitope coverage that helps when probing protein variants or post-translational modifications. Because the antibody is supplied unconjugated, investigators can pair it with HRP-, biotin-, or fluorophore-conjugated secondary reagents to match the requirements of their specific detection platform.

DAO (also annotated as AOC1) is a copper-dependent amine oxidase that breaks down histamine and putrescine, with prominent expression in the intestinal mucosa, kidney, and placenta. This makes the reagent relevant to research on histamine intolerance, gastrointestinal inflammation, and placental function. The rabbit host species ensures compatibility with the anti-rabbit secondary reagents already common in many immunology and cell biology laboratories.

For handling, store the vial frozen in working aliquots to limit freeze-thaw cycles and protect from light when paired with fluorescent secondaries. The unconjugated format also makes the antibody a candidate for custom labeling protocols, including biotinylation or direct conjugation to enzymes, when a particular detection chemistry is required.