Anti-Cytomegalovirus (CMV-pp65) Antibody

Cytomegalovirus pp65 is a 65 kDa tegument phosphoprotein that serves as a primary marker of active viral gene expression in infected cells, and the purified mouse monoclonal antibody from Antibodies.com is raised against this structural component for detection in research assays. The reagent is supplied as 100 µg of purified IgG2a(k) immunoglobulin without fluorophore or enzyme conjugation, leaving the choice of detection chemistry to the end user.

Validated applications listed by Antibodies.com include ELISA and western blot, covering quantitative serological measurements as well as confirmatory detection of pp65 protein bands in lysates from CMV-infected cells. Because pp65 is also the dominant target recognized by virus-specific CD8+ cytotoxic T lymphocytes, antibodies of this specificity are routinely deployed in studies of CMV latency, reactivation, and adaptive immune memory, including flow cytometry and intracellular cytokine staining workflows conducted for research use only.

The unconjugated, purified format is suitable for custom labeling with biotin, HRP, or fluorophores, and for affinity-based methods such as immunoprecipitation of pp65 from viral lysates or pull-down of pp65-bound cellular partners. Researchers working on transplant-associated CMV reactivation, congenital infection models, and vaccine candidates directed at tegument antigens rely on this antibody class as a reference reagent for tracking viral protein load and serostatus in experimental systems.

Aliquot the antibody stock on receipt to limit freeze-thaw cycling, and refrigerate the working vials; the purified IgG2a(k) preparation retains binding activity in ELISA and western blot when handled within standard short-term storage practice.