Anti-Cytokeratin 8 (G427) Antibody

This rabbit polyclonal antibody from Antibodies.com specifically recognizes Cytokeratin 8 (CK8/KRT8), a type II intermediate filament protein of approximately 52-54 kDa that is abundantly expressed in simple and glandular epithelia throughout the body. The unconjugated reagent is supplied as 100 µl at 1 mg/ml in phosphate buffered saline (pH ~7.2) with 15 mM sodium azide as preservative; it should be stored refrigerated at 4 °C and diluted directly into standard assay buffers as needed.

Validated applications include Western blot, immunohistochemistry, and immunofluorescence, supporting routine use as an epithelial cell marker in studies of carcinoma origin, circulating tumor cell identification, and epithelial-mesenchymal transition research. Reactivity with human, mouse, and rat samples broadens utility across cell culture models and tissue specimens.

Because Cytokeratin 8 is normally co-expressed with Cytokeratin 18 in most epithelial lineages, this reagent is frequently paired with anti-CK18 antibodies for co-staining experiments to confirm epithelial identity or to monitor filament integrity during apoptosis, when CK8 and CK18 are cleaved by caspases. The polyclonal format recognizes multiple epitopes on the target, which is advantageous when working with formalin-fixed paraffin-embedded tissue or denatured protein preparations.