Anti-Cytokeratin 8 Antibody

This unconjugated polyclonal reagent from Antibodies.com is raised in rabbit and targets Cytokeratin 8, a type II intermediate filament protein abundantly expressed in simple and stratified epithelial tissues. The antibody is supplied as 100 µL of liquid in a vial, ready for use across multiple immunoassay workflows including western blot, immunohistochemistry, immunofluorescence, and ELISA.

Researchers working in epithelial biology, tumor pathology, and cytoskeletal studies routinely rely on Cytokeratin 8 as a marker for epithelial differentiation and carcinoma characterization. Validated reactivity encompasses Human, Mouse, and Rat samples, supporting comparative work across standard model systems. Because the reagent is polyclonal, it can recognize multiple epitopes on the target, which is often advantageous when working with denatured preparations or when broader epitope coverage is desired, and the unconjugated format gives laboratories full flexibility to pair it with HRP- or fluorophore-conjugated secondary antibodies or custom labeling schemes.

For handling, the antibody should be stored refrigerated, protected from light, and shielded from repeated freeze-thaw cycles to preserve activity; aliquoting upon first use is recommended. Working concentrations should be optimized empirically for each application, and users are encouraged to consult the product datasheet for suggested starting dilutions in western blot and immunohistochemistry protocols. As a Primary Antibodies reagent intended for research use only, it fits naturally into standard immunodetection pipelines for epithelial cell culture, tumor tissue sections, and cytoskeletal fractionation studies, including investigations of epithelial-mesenchymal transition and circulating tumor cell characterization.