Anti-Cytokeratin 5 Antibody

This rabbit polyclonal reagent from Antibodies.com targets the intermediate filament protein Cytokeratin 5 (KRT5), a type II cytokeratin expressed in the basal layer of stratified and pseudostratified epithelia. The unconjugated antibody is supplied in a 100 µl vial volume and is validated for western blot, immunohistochemistry, immunofluorescence, and ELISA workflows across human, mouse, and rat sample types.

KRT5 is a tissue-specific marker for the basal layer of epidermis, hair follicle outer root sheath, prostate basal cells, and myoepithelial cells of breast and salivary gland. In pathology research, this Cytokeratin 5 Antibody helps distinguish squamous cell carcinoma and basal-like triple-negative breast cancer from luminal subtypes, and aids in differentiating mesothelioma (typically CK5-positive) from pulmonary adenocarcinoma, which is usually CK5-negative.

Because mutations in KRT5 are linked to epidermolysis bullosa simplex, the reagent is also referenced in dermatology research investigating keratin network integrity, skin blister formation, and the basal cell cytoskeleton. The polyclonal format allows recognition of multiple epitopes on the target protein, supporting reproducible staining of formalin-fixed paraffin-embedded tissue sections and reliable immunoblotting of whole-cell lysates from epithelial-origin samples.