Anti-Cytokeratin 18 Antibody

A primary antibody from Antibodies.com directed against Cytokeratin 18, a type I intermediate filament protein of approximately 48 kDa that is constitutively expressed by simple and glandular epithelia, hepatocytes, and most carcinoma cell lines. This unconjugated mouse monoclonal is provided as a 100 µL volume of purified immunoglobulin in 0.1 M Tris-Glycine buffer (pH 7.4) containing 150 mM NaCl, 0.2% sodium azide, and 50% glycerol, and has been validated for western blot and immunocytochemistry on human and non-human primate (monkey) samples. The reagent is widely used in research settings to characterize epithelial-mesenchymal transition, profile circulating tumor cells, and study caspase-mediated CK18 cleavage during apoptosis and liver injury, where the Asp238 neo-epitope generated by caspase-3, -7, and -9 serves as a quantitative biomarker of hepatocyte death. The 50% glycerol cryostabilant allows the solution to be stored refrigerated without freeze-thaw cycling, while the azide preservative protects against microbial contamination during routine benchtop handling.