Anti-Cytochrome c oxidase subunit 5A, mitochondrial Antibody

The COX5A antibody from Antibodies.com is a rabbit polyclonal raised against mitochondrial Cytochrome c oxidase subunit 5A, a nuclear-encoded component of Complex IV embedded in the inner mitochondrial membrane. Supplied as 100 µg of unconjugated immunoglobulin, this primary reagent is validated for western blot and immunohistochemistry on human samples and supports work in oxidative phosphorylation, mitochondrial bioenergetics, and the cellular response to hypoxia.

COX5A contributes to the assembly and stability of the cytochrome c oxidase catalytic core and is commonly used as an indicator of mitochondrial content. Published studies have linked altered COX5A expression to cancer metabolism, neurodegeneration, and ischemia-reperfusion injury, where shifts in its abundance often track changes between oxidative and glycolytic energy production.

Each vial should be stored aliquoted at -20°C, with working stocks refrigerated to limit freeze-thaw cycles, and the antibody protected from light until use. The unconjugated format lets researchers pair the reagent with HRP- or fluorophore-conjugated secondaries for chromogenic western blot or fluorescent IHC workflows. The product is frequently used alongside other OXPHOS complex subunit antibodies to profile respiratory chain integrity in human tissue and cell models.