Anti-Cytochrome b5 (G82) Antibody

This primary antibody from Antibodies.com is a rabbit polyclonal reagent raised against Cytochrome b5, specifically recognising the G82 epitope region. It is unconjugated and is validated for use in Western blot (WB), immunohistochemistry (IHC) and immunofluorescence (IF) applications, including ICC/IF.

The antibody was generated using a synthetic peptide corresponding to amino acids 51–100 of human Cytochrome b5 as immunogen, and it detects endogenous levels of Cytochrome b5 protein in samples from human, mouse and rat. It is supplied at 1 mg/ml in phosphate buffered saline (PBS) with 0.05% sodium azide at approximately pH 7.2.

According to the manufacturer, the target protein has a molecular weight of approximately 15 kDa, and the antibody has been affinity-purified from rabbit antiserum by affinity chromatography using the epitope-specific immunogen, with a purity greater than 95% as determined by SDS-PAGE.