Anti-Cystatin C (F122) Antibody

Raised in rabbit against the F122 epitope region of human Cystatin C, this unconjugated polyclonal antibody is supplied by Antibodies.com at 1 mg/ml in phosphate buffered saline containing 0.05% sodium azide, pH approximately 7.2. The reagent is provided as a 100 µl aliquot and is validated for Western blot (WB) detection of the ~13 kDa cysteine protease inhibitor encoded by the CST3 gene, with confirmed reactivity on human, mouse, and rat samples. Investigators working in renal physiology, cerebral amyloid angiopathy, neurodegeneration, and cardiovascular biomarker studies rely on anti-Cystatin C reagents because circulating cystatin C levels serve as a sensitive surrogate marker for glomerular filtration rate. Working dilutions should be titrated empirically against each lysate preparation to obtain clean signal near the expected molecular weight of roughly 13 kDa on reducing SDS-PAGE gels. For research use only, the unconjugated format allows flexible pairing with HRP, fluorescent, or biotinylated secondary reagents selected by the end user. Aliquot upon first use, store refrigerated between sessions, and avoid repeated freeze-thaw cycles to preserve antibody activity.