Anti-CYP7B1 Antibody

The CYP7B1 polyclonal reagent from Antibodies.com is an unconjugated rabbit IgG raised against the cytochrome P450 7B1 enzyme, supplied at 100 µL working volume. CYP7B1 catalyzes 6α- and 7α-hydroxylation of oxysterols and neurosteroid precursors such as pregnenolone and DHEA, positioning the antibody for studies of bile acid metabolism, neurosteroid signaling, and steroid hormone clearance.

Validated across western blot, immunohistochemistry, immunofluorescence, and flow cytometry workflows, the reagent recognizes human CYP7B1 and is suited to paraffin-embedded tissue sections, cultured cell lines, and isolated cells. Researchers probing hepatic and neural tissues commonly apply it to investigate CYP7B1 expression in hepatocellular carcinoma, spastic paraplegia type 5A, and congenital bile acid synthesis defects linked to oxysterol 7α-hydroxylase deficiency.

As an unconjugated primary, it accepts downstream labeling with HRP, biotin, or fluorophore conjugates chosen by the user, supporting flexibility across detection platforms. Aliquot upon receipt and store frozen to preserve activity through repeated freeze-thaw cycles; avoid repeated freeze-thaw of the working stock.