Anti-CYP2A6 Antibody

Antibodies.com offers a rabbit polyclonal IgG reagent directed against CYP2A6, a hepatic cytochrome P450 enzyme widely studied for its role in coumarin 7-hydroxylation and the oxidative clearance of nicotine to cotinine. The antibody is supplied at 200 µl in an unconjugated vial and is validated for Western blot on human and mouse samples, supporting research in drug metabolism, pharmacogenetics, and toxicology.

CYP2A6 is predominantly expressed in hepatocytes, where it contributes to phase I oxidation of xenobiotics, tobacco-specific nitrosamines, and several clinically prescribed medications. Probing CYP2A6 levels is relevant to studies of nicotine dependence, smoking behavior, and the inter-individual variability of drug response. The reagent also provides a useful entry point for investigating how cytochrome P450 enzymes are regulated under chemical or environmental challenge.

For Western blot, the antibody is used on standard denatured lysates and is typically paired with an HRP-conjugated secondary reagent and chemiluminescent detection. To preserve reactivity across experiments, aliquot the 200 µl volume upon arrival and store at -20°C, avoiding repeated freeze-thaw cycles that can compromise polyclonal performance.