Anti-CYP27A1 Antibody

This polyclonal antibody from Antibodies.com targets CYP27A1 (cytochrome P450 family 27 subfamily A member 1), the enzyme commonly referred to as mitochondrial sterol 27-hydroxylase. CYP27A1 is anchored in the inner mitochondrial membrane and catalyzes the hydroxylation of cholesterol and related sterol intermediates, acting as a key gatekeeper in the acidic (alternative) pathway of bile acid biosynthesis and contributing to vitamin D3 metabolism.

Raised in rabbit and supplied as an unconjugated reagent in a 100 µl vial, the antibody is validated for Western blot (WB) and immunohistochemistry (IHC). It cross-reacts with human and mouse antigen, supporting comparative studies of hepatic lipid handling, cholesterol homeostasis, and bile acid pathway activity. Researchers working on cerebrotendinous xanthomatosis (CTX), a lipid-storage disorder caused by CYP27A1 deficiency, as well as those examining broader questions of sterol and vitamin D metabolism, can apply this reagent to immunoblotting of whole-cell or mitochondrial lysates and to chromogenic or fluorescent staining of fixed tissue sections.