Anti-CYP27A1 Antibody

The CYP27A1 protein (cytochrome P450 family 27 subfamily A member 1), also called mitochondrial sterol 27-hydroxylase, is a key mitochondrial enzyme driving bile acid biosynthesis from cholesterol and contributing to vitamin D3 activation. This unconjugated rabbit polyclonal IgG reagent from Antibodies.com is supplied as a 100 µl liquid aliquot and is validated across western blot, immunohistochemistry, immunofluorescence, and ELISA workflows. Raised in rabbit host and affinity-purified, the reagent offers dependable performance for hepatic and extrahepatic research into cholesterol homeostasis, oxysterol metabolism, and bile acid pathway regulation.

Because the product is unconjugated, it can be paired with HRP-, biotin-, or fluorophore-labeled secondary reagents to support either chromogenic tissue staining or fluorescent imaging. Recommended uses include characterization of CYP27A1 expression in liver biopsies, hepatocyte cell lines, and adrenal or intestinal tissues where this enzyme plays known physiological roles. For antibody handling, aliquot upon receipt to limit freeze-thaw cycles, refrigerate at 4°C for short-term use, or freeze at -20°C for long-term retention of activity.