Anti-Cyclophilin F Antibody

A rabbit polyclonal antibody raised against Cyclophilin F (also called peptidyl-prolyl cis-trans isomerase F, encoded by the PPIF gene), supplied by Antibodies.com as an unconjugated primary antibody. Cyclophilin F is the mitochondrial isoform of the cyclophilin family and acts as a critical regulator of the mitochondrial permeability transition pore (mPTP), linking calcium overload, oxidative stress, and necrotic or apoptotic cell death. Researchers studying cardiac ischemia-reperfusion injury, neurodegeneration, and mitochondrial dysfunction use this reagent to profile CypD expression across human, mouse, and rat samples.

This reagent is provided as a 100 µl volume of polyclonal antiserum raised in rabbit, unconjugated for flexible downstream detection, and validated for Western blot (WB), immunohistochemistry (IHC), immunofluorescence (IF), and ELISA workflows. Its cross-species reactivity covers human, mouse, and rat tissues, supporting comparative studies of mitochondrial damage responses. The antibody binds Cyclophilin F rather than cytosolic cyclophilin isoforms such as CypA or CypB, enabling selective detection of the mitochondrial pool that gates mPTP opening under cellular stress.

Typical uses include confirming PPIF knockdown or overexpression in cell lysates by Western blot, localising CypD within mitochondrial networks by immunofluorescence alongside markers such as COX IV or TOM20, and characterising tissue distribution in sections of heart, brain, or liver by immunohistochemistry. The product ships as a vial of liquid antiserum intended for research use only, not for diagnostic or therapeutic applications.