Anti-Cyclin E1 Antibody

This rabbit polyclonal antibody targets Cyclin E1 (CCNE1), a regulatory cyclin that partners with cyclin-dependent kinase 2 to control the G1-to-S phase transition of the eukaryotic cell cycle. Supplied by Antibodies.com as a 100 µl unconjugated aliquot, the reagent is validated for western blot, immunohistochemistry, immunofluorescence, and ELISA workflows in human samples.

Cyclin E1 expression rises in late G1 and is rapidly degraded after S-phase entry, making this reagent useful for tracking proliferating populations in fixed or lysed material. Researchers studying oncogenesis frequently probe CCNE1 levels because gene amplification and protein overexpression are recurrent features of breast, ovarian, and endometrial carcinomas, and dysregulation correlates with genomic instability and poor prognosis.

The polyclonal host response yields recognition of multiple epitopes across the full-length Cyclin E1 protein, which can improve signal when the target is partially degraded or when post-translational modifications alter antibody binding sites. Storage at -20 °C in small aliquots helps limit freeze-thaw cycles and preserve binding activity for repeated experiments. Reactivity is reported for human samples, with the unconjugated format allowing flexible pairing with secondary reagents labeled to the user's preferred fluorophore or enzyme.