Anti-Cyclin E1 (Ab-395) Antibody

Manufactured by Antibodies.com and distributed through AFMS, this primary antibody is a rabbit polyclonal reagent raised against Cyclin E1, specifically recognising an epitope that includes the threonine 395 residue of the human protein. The immunogen is a synthetic peptide derived from human Cyclin E1 (corresponding to amino acids 361-410), and the antiserum has been affinity-purified by epitope-specific chromatography to maximise specificity. Supplied as 50 µl of liquid formulation in phosphate-buffered saline (pH 7.4) containing 150 mM NaCl, 0.02% sodium azide and 50% glycerol, the antibody is unconjugated and of the IgG isotype, with a predicted molecular weight of approximately 47 kDa. It is validated for Western blotting (WB), immunohistochemistry (IHC) and immunofluorescence (IF) applications, and the reactivity profile is restricted to human samples, making it suitable for studies of G1/S-specific cyclin-E1 (CCNE1) function in cell-cycle and cancer research. The host species is rabbit and the clonality is polyclonal, and the reagent is intended for research use only. For storage, the antibody should be kept at –20 °C, where it remains stable for at least one year, avoiding repeated freeze-thaw cycles to preserve binding activity. Cited in published work on cyclin E1 phosphorylation, this reagent provides a reliable tool for detecting total Cyclin E1 protein across a range of standard immunoassays.