Anti-Cyclin B1 (phospho-S126) Antibody

This rabbit polyclonal antibody from Antibodies.com specifically recognizes Cyclin B1 only when phosphorylated at serine 126, a modification associated with regulation of Cyclin B1 activation and mitotic entry during the cell cycle. The reagent is supplied as 100 µl of purified IgG at 1 mg/ml in phosphate buffered saline (approximately pH 7.2) with 15 mM sodium azide added as a preservative, and it is unconjugated for flexibility with secondary detection chemistries.

Validated for western blot and immunohistochemistry, the antibody was raised in rabbit and detects Cyclin B1 from human and mouse samples, supporting studies of mitotic progression, spindle assembly checkpoint activity, and aberrant proliferation in tumor tissue sections. The phospho-S126 epitope allows researchers to monitor the activated pool of Cyclin B1 separately from total protein levels, which is useful for tracking checkpoint recovery after DNA damage and for profiling cellular responses to kinase inhibitors that act during the G2/M transition.

For handling, store the vial refrigerated at approximately 4 degrees C for short-term use, or aliquot and freeze at -20 degrees C for longer storage; avoid repeated freeze-thaw cycles and protect the solution from light. The included 15 mM sodium azide inhibits microbial contamination, and the reagent is intended for research use only in laboratory settings, not for diagnostic or therapeutic procedures.