Anti-Cyclin A (W250) Antibody

Available from Antibodies.com, this rabbit polyclonal antibody is raised against Cyclin A (W250) and supplied unconjugated. It reacts with Human, Mouse and Rat samples and is validated for WB, IHC and IF applications (C0, S1, S3).

The antibody was generated using a synthetic peptide corresponding to amino acids 220-270 of Human Cyclin A as immunogen. It specifically detects endogenous levels of Cyclin A protein and has been affinity-purified from rabbit antiserum by affinity-chromatography using the epitope-specific immunogen, with purity greater than 95% as determined by SDS-PAGE. The target protein has a molecular weight of approximately 52 kDa. The product is formulated at 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approximately pH 7.2 (S1, S3).

Suggested starting dilutions are WB (1:1000-1:10000), IHC-P (1:50-1:500) and ELISA (1:500-1:10000). The antibody is stable for one year at -20°C from the date of shipment (S3).