Anti-Cyclin A Antibody

Cell cycle research requires reliable markers of S-phase progression and mitotic commitment, and this unconjugated rabbit polyclonal reagent from Antibodies.com targets Cyclin A, the conserved regulatory partner of CDK2 during DNA synthesis and of CDK1 at the G2/M transition. Supplied as 100 µl of antiserum, the antibody is validated for Western blot (WB), immunohistochemistry (IHC), and immunofluorescence (IF) workflows on human and mouse samples, where it detects endogenous Cyclin A protein at approximately 50-55 kDa depending on isoform.

Cyclin A expression rises as cells enter S phase and persists through G2, making this polyclonal reagent a standard tool for assessing proliferative indices in tumor sections, characterizing cell cycle re-entry in regenerating tissues, and probing S-phase kinetics in synchronized cell cultures. The unconjugated format allows free pairing with HRP-, biotin-, or fluorophore-conjugated secondary antibodies, supporting flexible detection across membrane-, slide-, and coverslip-based assays without restricting the laboratory to a pre-defined label or species-specific detection kit.

For reliable long-term performance, the 100 µl volume should be aliquoted upon receipt to limit freeze-thaw cycles, and the resulting single-use aliquots can be stored frozen under standard antibody conditions to preserve reactivity across ongoing oncology, developmental biology, and cell cycle projects.