Anti-Cyclin A Antibody

This unconjugated polyclonal primary antibody from Antibodies.com targets Cyclin A, a key cell cycle regulator that drives S phase entry and the G2/M transition through association with CDK1 and CDK2. Raised in rabbit, the reagent recognizes the conserved Cyclin A protein across human, mouse, rat, and monkey samples, supporting cross-species studies of proliferation, DNA replication, and mitotic control.

Validated for Western blot (WB), immunohistochemistry (IHC), immunofluorescence (IF), and ELISA workflows, the antibody is supplied as 100 ul of liquid stock in a single-use vial format and is provided unconjugated to allow flexible pairing with HRP, biotin, fluorophore, or alkaline phosphatase reporter chemistries. The polyclonal format enables recognition of multiple epitopes on Cyclin A, which commonly migrates near 50-60 kDa on SDS-PAGE, depending on isoform (CCNA1 versus CCNA2). Store aliquots at -20 degrees C and avoid repeated freeze-thaw cycles to preserve activity; the working solution can also be kept refrigerated for short-term use.

Typical research applications include studies of cell cycle checkpoints, oncogenesis and tumor proliferation markers, regenerative biology, and toxicology screens where Cyclin A expression is used as an indicator of actively dividing cells. For research use only.