Anti-CYB5 Antibody

Antibodies.com offers this unconjugated rabbit polyclonal antibody raised against Cytochrome B5 (CYB5), a heme-containing, endoplasmic reticulum-anchored protein that shuttles electrons to cytochrome P450 enzymes and supports drug metabolism, fatty acid desaturation, and cholesterol biosynthesis in eukaryotic cells. The reagent is validated for western blot and immunofluorescence workflows and detects the endogenous CYB5 protein in human, mouse, and rat samples, giving comparative studies cross-species coverage with a single reagent. Supplied as 100 µl of antiserum, the antibody is unconjugated, allowing laboratories to pair it with their preferred fluorophore, HRP, or biotin label depending on the detection platform in use.

The polyclonal format recognizes multiple epitopes across the CYB5 sequence, which generally produces stronger signal in western blot applications than monoclonal alternatives when target abundance is limited. For immunofluorescence, the unconjugated format is compatible with standard secondary antibody detection schemes on fixed cells and tissue sections. Refrigerate the vial on arrival and avoid repeated freeze-thaw cycles to preserve binding activity through the working shelf life of the reagent.