Anti-CXCR1 Antibody

This unconjugated polyclonal reagent, raised in rabbit against CXCR1 (C-X-C chemokine receptor 1, also known as IL-8RA or CD181), is supplied through Antibodies.com as a 100 µL vial at 1 mg/mL in phosphate buffered saline (PBS) containing 0.05% sodium azide, approximately pH 7.2. It is intended for research use only and is validated for western blot detection of human, mouse, and rat CXCR1.

CXCR1 is a seven-transmembrane G protein-coupled receptor that binds the chemokine CXCL8/interleukin-8 and drives neutrophil chemotaxis, calcium flux, and integrin activation during acute inflammation. Researchers studying the receptor interrogate neutrophil recruitment in sepsis, acute lung injury, inflammatory bowel disease, and rheumatoid arthritis models, and probe the same target in oncology workflows where CXCR1 supports tumor cell proliferation, angiogenesis, and metastasis in breast, lung, colon, and melanoma systems. The antibody is therefore commonly used to track receptor expression in cell lysates and tissue homogenates by immunoblotting, complementing parallel flow cytometry or immunohistochemistry readouts in the same project.

For western blot, resolve samples on SDS-PAGE, transfer to PVDF or nitrocellulose, block in 5% non-fat milk or BSA, and incubate with the primary antibody at an empirically optimized dilution in TBS-T. Aliquot on receipt and store at -20°C to limit freeze-thaw cycles; the 0.05% sodium azide protects against microbial contamination during routine liquid handling. Keep vials protected from light and avoid prolonged exposure to room temperature. Recommended handling matches general IgG storage guidance for research-grade polyclonal reagents.