Anti-Cullin 1 Antibody

This unconjugated rabbit polyclonal antibody from Antibodies.com targets Cullin 1, a core scaffold subunit of the SCF (Skp1–Cullin–F-box) E3 ubiquitin ligase complex. Cullin 1 nucleates SCF assembly and recruits substrate recognition F-box proteins, making the protein a focal point in studies of cell cycle progression, particularly G1/S transition via p27^Kip1 turnover, and broader ubiquitin-mediated proteasomal degradation pathways.

Supplied as a 100 µl vial of polyclonal antiserum raised in rabbit, the reagent is validated for Western blot, immunohistochemistry, and immunofluorescence workflows. The antibody is reactive with human Cullin 1, supporting detection in routinely cultured human cell lines and fixed tissue specimens used in cancer biology and signal transduction research.

Because Cullin 1 is frequently overexpressed or dysregulated across multiple tumor types, this antibody is commonly used to examine SCF complex integrity, cullin neddylation status, and proteasome-dependent turnover of downstream substrates. Researchers employ it to map Cullin 1 distribution in tissue sections, monitor expression changes after small-molecule inhibitor treatment, and probe interactions with F-box partners by co-immunoprecipitation.

For handling, aliquot the vial upon first thaw to limit freeze-thaw cycles and store refrigerated for short-term use; long-term storage at -20 °C in working aliquots is recommended to preserve antibody titer.