Anti-CUL2 Antibody

Cullin-2 (CUL2) is an approximately 82-84 kDa scaffold protein that assembles with Elongin B/C and substrate adaptors such as pVHL to form a multi-subunit CRL2 E3 ubiquitin ligase complex, targeting specific proteins for proteasomal degradation and regulating pathways including hypoxia sensing, cell cycle progression, and the DNA damage response.

This unconjugated rabbit polyclonal IgG reagent from Antibodies.com recognizes endogenous CUL2 in human and mouse samples and is supplied as 200 µl of liquid in a single vial, with a stock concentration of approximately 1 mg/mL in PBS buffer containing 0.1% sodium azide and 50% glycerol. The antibody was affinity-purified from rabbit antiserum by epitope-specific affinity chromatography.

Validated for western blot detection of endogenous CUL2, immunohistochemistry on formalin-fixed tissue sections, and immunofluorescence staining of cultured cells, this reagent supports studies of CUL2 expression, complex assembly, and subcellular localization across diverse experimental systems. Its polyclonal format offers broader epitope coverage than a monoclonal reagent, useful when probing for variant isoforms or when target epitopes may be partially masked by post-translational modification.

For best stability, store at -20 °C and avoid repeated freeze-thaw cycles by preparing single-use working aliquots. The vial is shipped refrigerated on wet or dry ice and the product is intended for research use only.