Anti-CTNNB1 (phospho S33) Antibody

Phosphorylation of β-catenin at serine 33 marks a key regulatory event in the canonical Wnt signaling cascade, where it primes the protein for recognition by the β-TrCP ubiquitin ligase complex and subsequent proteasomal degradation. This rabbit polyclonal IgG antibody from Antibodies.com specifically detects the phosphorylated S33 epitope of human CTNNB1, enabling researchers to monitor Wnt pathway activity and destruction complex function in cell lysate preparations.

Provided as a 50 µl aliquot of unconjugated antiserum, the reagent is validated for western blot workflows and supports studies comparing basal versus ligand-stimulated β-catenin phosphorylation, evaluating GSK-3β inhibition, or characterizing crosstalk between Wnt and other signaling networks. The polyclonal format offers recognition of multiple epitopes surrounding the phospho-S33 site, which can improve signal robustness in blotting applications.

Researchers working with human cell lines and primary cultures will find this reagent useful for time-course experiments following Wnt3a stimulation, lithium chloride treatment, or small-molecule modulation of casein kinase 1 and GSK-3β activities. Store aliquots to limit freeze-thaw cycles and protect from light when not in use.