Anti-CTNNB1 (phospho S33) Antibody

Phosphorylation of beta-catenin at serine 33 flags the protein for ubiquitin-dependent degradation and serves as a direct readout of glycogen synthase kinase 3 (GSK-3) activity within the canonical Wnt signaling cascade. This unconjugated rabbit polyclonal IgG reagent from Antibodies.com is raised against the phospho-S33 epitope of CTNNB1 and detects the modified human target by Western blot.

Supplied as a 200 µl vial, the antibody recognizes multiple epitopes around the modified serine, supporting robust signal under denaturing SDS-PAGE conditions. Researchers commonly apply it to lysates from colorectal cancer cell lines, hepatocellular carcinoma models, and stem cell differentiation time courses in which Wnt pathway modulation shifts beta-catenin stability. The polyclonal IgG format broadens the recognition footprint at this single phospho-residue compared with most monoclonal alternatives.

To preserve phospho-epitope binding, aliquot the antibody upon receipt and avoid repeated freeze-thaw cycles; short-term refrigerated storage is appropriate. Intended for research use only.